Tags & Description
What are the structures and organelles of a typical eukaryotic cell?
plasma membrane, nucleus, nucleolus, nuclear membrane, smooth endoplasmic reticulum, nuclear membrane pore, rough endoplasmic reticulum, lysosome, mitochondrion, cytosol, filamentous cytoskeleton (microtubules), Golgi apparatus
What are the major differences between a prokaryotic and eukaryotic cell?
prokaryotes have no defined nucleus or membrane-bound organelles, eukaryotes are usually the cells of multicellular organisms and have membrane-bound organelles
What are the main features and functions of plasma membranes? Focus on lipid bilayers and proteins/glycoproteins of plasma membranes.
hydrophobic and hydrophilic moiety
cholesterol enhances mechanical stability and reduces membrane permeability
membrane proteins responsible for functions
carbohydrates present as glycolipids and glycoproteins
glycocalyx: a glycoprotein covering that surrounds the cell membranes for cell-cell recognition, communication, and intercellular adhesion
What are the main features and functions of cytoplasmic matrices? Focus on key proteins of the three types of cytoplasmic matrices and their relative position and size.
provides structural support to define shape
framework for positioning of organelles
network to direct the movement of materials and organelles within the cells
means of independent locomotion for specialized cells
pathway for intracellular communication
possible transfer of RNA and DNA
cytoskeleton components
microtubules: smallest
protein: tubulin
intermediate filaments
protein: various fibrous proteins
microfilaments: largest
protein: actin
Structural arrangement of the matrix influences metabolic pathways such as…
glycolysis
hexose monophosphate shunt (pentose phosphate pathway)
glycogenolysis
fatty acid synthesis
facilitate interactions between enzymes and molecules
What are the main features and functions of mitochondria?
double membrane surrounds matrix
outer membrane is relatively porous
inner membrane is selectively permeable and the site of electron transport chain
site of TCA cycle and fatty acid oxidation
contains DNA through maternal inheritance
can divide and change in size
TCA cycle
What are the main features and functions of cell nuclei?
largest organelle
surrounded by nuclear envelope
nuclear structural components
contains DNA
cell genome is the entire set of genetic information
protein synthesis facts
not all genes are expressed at the same time
not all genes are expressed to become proteins
not all genes are expressed in the nucleus
DNA sequences are not always equivalent to genes
What is the major function of a nucleolus?
site of rRNA transcription and processing
ribosome assembly and synthesis
Key steps and procedures of transcription and replication and where they occur
DNA replication
duplex DNA unwinds and nucleotides are added to each strand to make two sets
in the S-phase of the cell cycle
transcription
mRNA created from sequence of one DNA strand (sense strand)
genes
introns: intervening sequences
exons: containing coding sequences
coding exons, but not intron, sequences to become protein
transcription of a gene in the nucleus results in the synthesis of a strand of mRNA
Key steps and procedures of translation and where they occur
in translation and elongation, the mRNA strand leaves the nucleus, binds to ribosomes, and directs protein translation with the help of tRNA subunits and their associated amino acids. This elongation process results in the production of a polypeptide strand
mRNA codes for amino acid one by one to form protein
mRNA is synthesized in nucleus, then moves to RER in cytoplasmic matrix
codon: a 3-base sequence codes for one amino acid
tRNA brings amino acids to mRNA
tRNAs have their specific 3-base anticodon RNA sequence to match its codon, and carry the correct amino acids
the 61 different tRNAs (see the genetic code) are preloaded with their unique amino acid and wait for the next codon to come
elongation
after amino acids are positioned, peptide bonds are formed between them
“nonsense” (stop) codons signal the end of translation
only a portion of the genes are expressed in cells of a given organ
regulation of gene expression occurs at various levels
transcription
mRNA processing (combinations of exons)
translation
exons during transcription
contain coding sequences
introns during transcription
intervening sequences
What is DNA replication?
the process by which the genome’s DNA is copied into cells
Why is DNA replication needed?
to ensure that when a cell divides, daughter cells have a full copy of the genome and therefore, successful inheritance of genetic traits
What are the main features and functions of endoplasmic reticulum?
network of membranous channels used to communicate from the innermost part of the cell to the exterior
protein quality control
types
rough ER (studded with ribosomes): protein synthesis
smooth ER: lipid synthesis
sarcoplasmic reticulum (SER) in muscle: calcium ion pump
*ribosomes
on ER: for extracellular and organelle destination
free in cytosol: for cytosolic proteins
What are the main features and functions of Golgi complex?
protein trafficking and sorting
how do proteins know their final destinations? add oligosaccharides, lipids, or a few amino acids
4-8 cisternae (stacks)
tubular networks at either end
cis-Golgi network: entrance
trans-Golgi network: exit
connected to ER by transport vesicles
What are the main features and functions of lysosome?
enzyme-filled organelles
lysosomes serve as cell’s “digestive system”
capable of degrading proteins, polysaccharides, nucleic acids, and phospholipids
What are the main features and functions of peroxisome?
enzyme-filled organelles
site of oxidative catabolic reactions
ex: oxidation of ethanol to acetaldehyde
using H2O2 as a substrate through catalase
What are the three types of protein receptors?
ion channel receptors, GPCRs, and enzyme-linked receptors
those that generate internal chemical signals
those that function as ion channels
those that internalize stimuli
receptors info
internal chemical signal
ex: 3’-5’-cyclic adenosine monophosphate (cyclic AMP, cAMP)
ion channel
ex: receptor for acetylcholine- to open and allow for Na+ entry
the proton pump
internalize stimulus: Clathrin-coated pit
variety of biologically active molecules
several hormones
receptors
modify cell’s response to environment
enzymes
catalysts for biochemical reactions within cells
ligands
proteins or chemicals that stimulate and attach to receptors
receptors steps
ligand binds with its receptor on the cell membrane
ligand and receptor move into a clathrin-coated pit
pit closes off and forms a clathrin-coated vesicle
the vesicle forms an endosome
ligand can be used by the cell or undergo lysosomal degradation
receptor is recycled to the surface of the cell membrane
ex: virus; bacterial toxins
catalytic proteins (enzymes)
functionality depends on protein and prosthetic group( non-protein part) or coenzyme
enzyme’s active site has high specificity
velocity defined by an equilibrium constant
Km (Michaelis constant)
concentration of substrate when reaction is at half of maximum velocity
reversibility
most are reversible
enzyme (E) + substrate (S) <→ E-S complex → E + products
mechanisms of regulation
covalent modification of enzymes: at active site
modulation of allosteric (change binding affinity to substrate upon effector binding, with conformational changes) enzymes
ex: phosphofructokinase, inhibited by citrate but stimulated by AMP/ADP
increase in enzyme concentration by induction
enhance the amount of proteins
vitamin D can increase Ca bioavailability
six classes of enzymes
oxidoreductases: reactions in which one compound is oxidized, another reduced
Gpx1
transferases: functional group transferred from one substrate to another
adenyl cyclase
hydrolases: hydrolysis of bonds between carbon and other atoms by adding water
lyases: cleavage of C-C, C-S, and C-N bonds (no hydrolysis/O-R)
isomerases: interconversion of optical or geometric isomers (R vs. L)
ligases: catalyze formation of C and other bonds (O, S, N, others)
examples of using proteins as clinical markers of diseases
tumor markers
substances produced as a result of a malignant disease
telomerase
enzymes and isoenzymes, hormones, protein antigens such as carcinoembryonic antigen (CEA; a glycoprotein in association with intestinal cancer), and products of oncogenes
oncogenes: mutated genes that encode abnormal, mitosis-signaling proteins that promote unregulated cell division
What is apoptosis?
programmed cell death
What is senescence?
permanent termination of cell proliferation
also known as cell aging
during the aging process, most somatic cells take longer and longer to proliferate and eventually reach senescence
causes
aged cells
persistent DNA damage response due to shortened telomeres (located at chromosome ends)
acute stress
oxidative stress; acute damage to DNA, protein, or lipids
Know what biological energy is about (energy-requiring processes, high energy phosphate bonds, and energy-requiring processes).
ATP: major storage form of energy in cells
energy needed for:
physical exertion
anabolism
active transport
transfer of genetic information
enzymatic reactions
energy comes from macronutrients (and alcohol!)
transferred from one form to another
heat by combustion vs chemical energy inside a cell
units of energy (cal, kcal, J, kJ)
1 cal=4.18J, or 1kcal=4.18kJ
free energy (G)
potential energy released from chemical bonds of nutrition