adv micro exam 2

The best way to isolate Listeria from a mixed culture is to use:

Upon examination, a 7-day-old infant is found to have mucopurulent discharge from his eye. A Giemsa stained smear of his eye scraping shows intracellular inclusions in epithelial cells. The MOST probable organism is which of the following?

An infant was admitted to the hospital with symptoms of meningitis. A lumbar puncture was performed and cultures were ordered. What is the best tube to be sent to Micro for culture and susceptibility testing?

Clue cells are found on a direct smear of a vaginal specimen. This indicates a possible infection with:

Endemic trachoma and genital infections are caused by:

The most common type of organisms found in the normal intestinal tract is:

Which of the following organisms causes hemolytic uremic syndrome in children associated with consumption of under-cooked beef?

Hyphae which has become divided into a chain of cells by the formation of transverse walls at regular intervals are described as being:

Which of the following is a sexual spore?

Listeria may be differentiated from Group B Beta hemolytic Streptococci by the following tests

A purulent CSF drawn from and 18 year old male reveals the following: WBC: 200/microliter, 98% neutrophils, protein: 80 mg/dl, Glucose: 20 mg/dl. These findings are MOST consistent with

Non-gonococcal urethritis (NGU) can be caused by certain species of which of the following:

You have set up biochemical tests on a non-lactose fermenting colony from Hektoen agar on a stool culture. After 4 hours, the urea slant is bright pink. Select the most appropriate action:

Which genera of the Enterobacteriaceae are known to cause diarrhea and are considered enteric pathogens?

In the study of molds it is often necessary to observe the relationship between the reproductive structures and mycelium. This is best accomplished by:

The slide culture technique is useful to:

The best procedure to differentiate Listeria monocytogenes from Corynebacterium species is:

The following statement BEST describes the results of a CSF from a patient with bacterial meningitis:

Soft chancre or chanchoid is due to infection with

E. coli 0157:H7:

Microscopically, Vibrio appears as a:

The thick-walled conidia formed from a pre-existing hyphal cell which detaches from the hyphae is a(n):

Fungus cultures should be incubated at which of the following temperatures for maximum recovery:

A CSF was received into your microbiology lab. Upon examining the patient information on the request from and comparing it to the information on the specimen itself, a difference in names is noted. The appropriate course of action would be to:

A colony on blood agar from a vaginal specimen looks like group B Strep. However, the colonies are catalase positive and gram stain reveals a gram positive rod. It has enhanced umbrella motility at room temperature and grows at 4°C. It probably is:

"Clue cells" indicating infection with Gardnerella vaginalis may be observed in which of the following?

Normal flora of the female genital tract (of childbearing age) includes heavy amounts of:

Which of the following tests best differentiate Shigella species from E. coli?

A mass of threadlike filaments, branched or composing a network, which constitute the vegetative structure of a fungus is:

In the study of molds it is often necessary to observe the relationship between the reproductive structures and mycelium. This is best accomplished by:

A woman who had recently returned from a vacation in Mexico was admitted to the hospital. She was febrile and complained of flu-like symptoms. Her case history revealed that she had eaten cheese that had been made from unpasteurized milk while on vacation. The most likely etiologic agent in this case would be:

The MOST common cause of bacterial meningitis in newborns is:

Bacterial pathogens for newborns, which are always looked for on a routine genital culture from the mother, are:

When examining stool cultures for Enterohemorrhagic E. coli 0:157 H7, select the media used and colony characteristics of this organism:

A lactophenol cotton blue tease prep is prepared on a fungal colony. Upon examination no intact sporulating structures can be found. Select the most appropriate technique which will easily show intact sporulating structures.

The most appropriate step to visualize intact sporulating structures on a fungus would be:

Small, milky white, beta hemolytic colonies that are catalase negative grow from the CSF of a one-week-old baby. Select the most appropriate work-up:

Which virus is associated with genital warts and produce lesions of skin and mucous membranes?

A small, beta-hemolytic, catalase positive colony has been isolated from a vaginal culture. Gram stain shows gram positive rods and umbrella motility in semi-solid agar. This is most descriptive of:

Safety practices in Mycology include

The MOST common pathogens causing meningitis in older children and adults are:

Which of the following are characteristics of Listeria monocytogenes:

Corn meal agar is most useful in:

Select the method used to screen for E. coli 0157:H7 in stool specimens:

Which of the following is/are characteristic of Chlamydia trachomatis

A fecal specimen is inoculated with Hektoen enteric agar. Blue-green colonies are isolated. Biochemical screening produces the following key characteristics. TSI: K/A no gas Motility: Non motile Lysine Decarboxylase: Negative Urease: Negative The isolate MOST probably belongs to which of the following genera?

In working up a stool culture you carefully pick up four different suspicious colony types and inoculate them to TSI, LIA, and urea. The next day the following results are noted. Which should be worked up further as a possible Salmonella or Shigella?

Treatment of Vibrio cholera is imperative because of:

A stool pathogen, which requires a media with increased salt such as TCBS, is:

Streptococcus agalactiae is most noted as a cause of:

A stool specimen was received in the lab and the clinical history of the patient indicated possible infection with Vibrio. Which additional medium should be inoculated

A purulent CSF drawn from and 18 year old male reveals the following:WBC:200/μl, 98% neutrophils Protein: 80 mg/dl Glucose:20 mg/dl These findings are MOST consistent with:

The organism, which may be confused with Corynebacterium species (diphtheroids) on a Gram stain smear, is:

An opportunistic yeast, which never forms mycelia or pseudohyphae, does not have a capsule, and assimilates only glucose and trehalose is most likely

The specific type of conidium produced by asexually reproducing yeast is the:

Serious infection by yeast may occur in diabetics, young children, or infants and the immunosuppressed host. What is the most probable causative agent?

A white, powdery mold with brown reverse pigment is isolated on Mycosel agar in 5 days. Microscopic morphology demonstrates septate hyphae, globose microconidia in grape-like clusters and smooth pencil shaped multi celled macroconidia. Select the most appropriate techniques for definitive identification:

The hair penetration test is utilized primarily to differentiate the following organisms:

The Dermatophytes infect which of the following:

Sporothrix schenckii has the following characteristic

This organism was isolated from an inflammatory lesion of the skin from the scalp of a young male athlete. Pictured below is the organism on sab dex agar and a lactophenol blue mount. Based on these observations, what is the presumptive identification of the fungus?

Which of the following causes Chromomycosis:

Which of the following morphological features would correspond to Phialophora sp.?

Which of the following on direct exam can differentiate Eumycotic mycetoma from actinomycotic mycetoma:

The slide culture technique is useful to:

A definitive method to fully identify a yeast is:

This organism was collected from an immunocompromised patient with prolonged antibiotic use. The images below are from the cornmeal slide culture of this organism. What is the identification of this organism?

Pictured below is an organism isolated from a lesion of the mouth. What is the presumptive identification of this organism?

The morphologic structures which identify Candida albicans and distinguish it from other Candida species are:

The species of fungus causing epidemic ringworm infection in children in the US is:

A fungus presumptively identified as a Trichophyton is inoculated to T agars. T1 and T2 show scant growth. T3 and T4 show good growth. The organism is:

Species of Cladosporium, Phialophora, and Fonsecaea can be differentiated from each other by:

Illustrated in this image is the fruiting head of one of the fungal agents of chromomycosis. Which of the following represents the type of sporulation illustrated here?

The thick-walled conidia formed from a pre-existing hyphal cell which detaches from the hyphae is a(n):

The yeast characterized by "giant hyphae" on cornmeal is:

The genus of Dermatophytes characterized by the thick-walled, echinulate, spindle shaped macroconidia is:

An organism, which usually causes a lymphatic infection after an initial prick with a rose thorn, is which of the following:

A carnation grower in Colorado developed an ulcerated lesion on his hand. With time the infection spread up the lymphatics resulting in several lesions along his arm. The fungus most likely to be responsible for this disease is:

A mass of threadlike filaments, branched or composing a network, which constitute the vegetative structure of a fungus is:

A yeast, which produces pink to red colonies, is:

A yeast-like organism has been isolated from a blood culture. No mycelium was produced on corn meal agar, inositol was assimilated, and urease was produced at 35°C. This organism is:

If a single budding, yeast surrounded by a wide gelatinous capsule is found in an India ink preparation of the spinal fluid, or is + by Cryptococcal Antigen EIA, the organism is most likely:

A dermatophyte, which produces smooth, thin-walled macroconidia singly or in clusters and which does not produce microconidia is:

From a skin scraping culture of tinea pedis, a colony with a very white and powdery surface and patchy reddish-brown reverse pigment has grown. Microscopically, numerous microconidia (en grappe) and spiral hyphae are seen. Hair penetration test is positive. The most likely identification is:

The tissue form of chromomycosis is:

Given the image below, what is the presumptive identification of this organism?

Which of the following best describes the size and staining characteristics of yeast cells on a gram stain?

Tinea versicolor is caused by which of the following fungi:

A fungus cultured from a case of tinea corporis produces thick-walled, spindle-shaped macroconidia, with 4-6 cells. The colony is tan colored and powdery with a neutral reverse. Which of the following is it most likely to be:

This colony was obtained from a sputum fungal culture from a construction worked with a lung abscess. What is the identification of this organism?

A black mold is isolated from a tissue specimen. Microscopic morphology is simple conidiophores containing long chains of small oval conidia. The identification of this organism is:

Candida albicans is responsible for a wide variety of diseases and one fourth of all deaths caused by fungi in the US. A factor contributing to this situation is the fact that:

Fungal skin infections may be due to:

Agents of chromomycosis are:

Yeast recovered from the urine of a catheterized patient receiving chemotherapy for cancer gave the following results: Germ tube + Blastospores + Pseudohyphae + Arthospores 0 Chlamydospores + What further testing is necessary?

The species of Trichophyton which produces pencil shaped macroconidia, a deep red reverse pigment and tear dropped microconidia in thyrses is:

This is an image from a 4-day old culture form a "ringworm" cutaneous infection. With this lactophenol cotton blue stain, what is the identification of this organism?

A fungus isolated from a case of "Athlete's Foot" is found to produce urease and is hair penetration positive. The most likely identification is:

The following tests are utilized to distinguish Cryptococcus neoformans from other cryptococccus species:

A yeast-like organism has been isolated from a blood culture. No mycelium was produced on corn meal agar, inositol was assimilated, and urease was produced at 35°C. This organism is: